Automated purification and quantification of oligonucleotides.

A Biotechnological Perspective on The Affinity Magnetic Separation and Purification Based on Oligonucleotides

The rapidly growing field of biotechnology has created a critical need for simple, fast andhigh-throughput processes for the separation and purification of biomolecules from biologicalmatrices. In recent years, several bioseparation techniques have been proposed as advancedalternatives to the classical separation methods. These modern processes emphasize ultrahighselective and sensitive analysi...

Synthesis and purification in a single column on a high-throughput automated oligonucleotide production system.

Oligonucleotides are synthesized and purified in a single column filled with a mixture of nucleoside-loaded and underivatized, high-cross link, non-swelling polystryene beads. A new oligonucleotide production system has been developed to completely automate-the entire process of sequence entry, phosphoramidite synthesis, cleavage, deprotection, purification, quantitation, and sample collection....

Semi-quantitative segmental perfusion scoring in myocardial perfusion SPECT: visual vs. automated analysis

Introduction: It is recommended that the physician apply at least a semi-quantitative segmental scoring system in myocardial perfusion SPECT.  We aimed to assess the agreement between automated semi-quantitative analysis using QPS (quantitative Perfusion SPECT) software and visual approach for calculation of summed stress  score (SSS), summed rest score (SRS) and summed difference score (SDS). ...

Specific Inhibition of the Expression of the Promyelocytic Leukemia (PML) Protein by Anti-Sense Oligonucleotides

In the present study, using anti-sense oligonucleotides the inhibition of expression of the PML protein hasbeen investigated. The anti-sense oligonucleotides were designed against the translation initiation site ofthe PML gene, and their effects were investigated on cellular growth and DNA synthesis. Incubation of normalhuman fibroblast cells with the anti-sense oligonucleotid...

Chemical Synthesis and Purification of Oligonucleotides